human hepatoblastoma cell line hepg2  (DSMZ)

Journal: BMC Cancer

Article Title: SKP2 ubiquitylation modifies IDH1 to regulate hepatoblastoma cell cycle and glucose metabolism

doi: 10.1186/s12885-025-14644-5

Figure Lengend Snippet: SKP2 inhibitors can inhibit the growth of hepatoblastoma and regulate cell cycle. A Western blotting was used to analyze the effect of SKP2 inhibitor. B CCK-8 was used to detect the IC50 of each cell line to SKP2 inhibitor and the growth curve of each cell line treated with SKP2 inhibitor for 3 days. C Cell cycle diagram of HepG2 cells in the presence of 25µM and 35µM SKP2 inhibitors

Article Snippet: Two types of human hepatoblastoma cell lines HepG2 and Huh6, and human hepatocellular carcinoma cell lines Huh7, as well as the normal hepatocyte cell line THLE-2, were purchased from ATCC (Manassas, USA).

Techniques: Western Blot, CCK-8 Assay

Journal: BMC Cancer

Article Title: SKP2 ubiquitylation modifies IDH1 to regulate hepatoblastoma cell cycle and glucose metabolism

doi: 10.1186/s12885-025-14644-5

Figure Lengend Snippet: SKP2 inhibitors can inhibit the growth of hepatoblastoma and regulate cell metabolism. A HepG2 and Huh6 migration ability was detected by Wound-healing assay. (B)Ability of cells to form colonies after 14 days of inhibitor treatment. B HepG2 and Huh6 cells after different treatments, Transwell assay was used to detect the migration and invasion ability of cells. C Histogram of glucose metabolism in cells treated with SKP2 inhibitor. D Effects of SKP2 inhibitors on subcutaneous tumors. E HE an-d immunohistochemical maps of the SKP2 inhibition group and the normal group. Scale bar = 100 μm. Statistical differences are indicated as follows: * p < 0.05, * * p < 0.01, * * * p < 0.001, * * * * p < 0.0001

Article Snippet: Two types of human hepatoblastoma cell lines HepG2 and Huh6, and human hepatocellular carcinoma cell lines Huh7, as well as the normal hepatocyte cell line THLE-2, were purchased from ATCC (Manassas, USA).

Techniques: Migration, Wound Healing Assay, Transwell Assay, Immunohistochemical staining, Inhibition

Journal: BMC Cancer

Article Title: SKP2 ubiquitylation modifies IDH1 to regulate hepatoblastoma cell cycle and glucose metabolism

doi: 10.1186/s12885-025-14644-5

Figure Lengend Snippet: Molecular docking of IDH1 with SKP2. A RMSD curve of the complex, Rg curve, RMSF curve of the protein, SASA curve of the complex, and hydrogen bond change curve of the complex. B Gibbs free energy topography of the complex. IDH1 protein expression after 2 h, 4 h and 8 h of MG132 and untreated HepG2 cells treated with CHX, respectively. Co-IP assay was used to verify that SKP2 ubiquitinated IDH1 in hepatoblastoma cell lines. SKP2 ubiquitylation modifies IDH1 and then degradation of the ubiquitinated IDH1 protein by the proteasome

Article Snippet: Two types of human hepatoblastoma cell lines HepG2 and Huh6, and human hepatocellular carcinoma cell lines Huh7, as well as the normal hepatocyte cell line THLE-2, were purchased from ATCC (Manassas, USA).

Techniques: Expressing, Co-Immunoprecipitation Assay

Journal: BMC Cancer

Article Title: SKP2 ubiquitylation modifies IDH1 to regulate hepatoblastoma cell cycle and glucose metabolism

doi: 10.1186/s12885-025-14644-5

Figure Lengend Snippet: Moderate IDH1 inhibitors can lead to the growth of hepatoblastoma. A CCK-8 was used to detect the effect of different concentrations on cell proliferation and the proliferation ability of HepG2 cells in 0.4µM IDH1 inhibitor and non-treatment groups within 48 h. B Western blotting was used to analyze the effect of IDH1 inhibitor. C HepG2 cells after different treatments. D Transwell assay was used to detect the migration and invasion ability of cells. E Cell migration ability was detected by Wound-healing assay. Ability of cells to form colonies after 14 days of inhibitor treatment. F Histogram of glucose metabolism in cells treated with IDH1 inhibitor. Statistical differences are indicated as follows: * p < 0.05, * * p < 0.01, * * * p < 0.001, * * * * p < 0.0001

Article Snippet: Two types of human hepatoblastoma cell lines HepG2 and Huh6, and human hepatocellular carcinoma cell lines Huh7, as well as the normal hepatocyte cell line THLE-2, were purchased from ATCC (Manassas, USA).

Techniques: CCK-8 Assay, Western Blot, Transwell Assay, Migration, Wound Healing Assay